| Titre : | Antioxidant activity evaluation of three medicinal plants of the Asteraceae family and their binary combination |
| Auteurs : | Souhila BENALI, Auteur ; SIDI IKHLEF Amel, Directeur de thèse |
| Type de document : | texte imprimé |
| Editeur : | Dr. Moulay Tahar Université Saida, Faculté des Sciences Naturelles et de la Vie, 2024/2025 |
| Format : | 41 p / 29 CM |
| Accompagnement : | CD |
| Langues: | Anglais |
| Langues originales: | Anglais |
| Catégories : | |
| Mots-clés: | phytochemicals, Antioxidant activity ; Anacyclus pyrethum ; Cichorium intybus ; Silybum marianum ; antioxidant activity ; binary mixtures. |
| Résumé : |
Plants with medicinal properties play an increasingly important role in food and
pharmaceutical industries for their functions on disease prevention and treatment. This study characterizes the qualitative Phytochemical contents and antioxidant activity of three plants, including the roots of Anacyclus pyrethrum(AP), stemsofCichorium intybus(CI), as well as the seeds of Silybum marianum(SM). in addition, we studied the antioxidant effects of their binary mixtures. The analysis of the phytochemicals qualitatively showed the existence of alkaloids in the three plants, flavonoids and catcholic tannins in CI and SM. in addition we found the anthocyanins and Starch in CI and SM extracts respectively. However, Coumarins, Reducing Compounds, Saponins and gallic tannins were absent in all plants. Results of antioxidant activity revealed that the hydro-methanolic roots and stems extracts of AP and CI respectively, displayed the highest antiradical effect with IC50 393 and 392 µg/ml respectively, however SM extract seeds presented low antioxidant activity with IC50 1500.2 µg/ml. in addition we found that AP and CI increases the antiradical effect of SM. While the mixture of the extract AP and CI causes a decrease in antioxidant activity compared to the two individual plants. Our study suggests antioxidant interactions in a mixture can influence its antioxidant activity positively or negatively. Further studies are desired on the identification of biochemical compounds of these plants and in vivo studies should be performed to confirm the results obtained so far. |
| Note de contenu : |
PART I. NTRODUCTION ............................................................................................... 2
PART II. BIBLIOGRAPHIC SYNTHESIS ................................................................. 3 II.1 Medicinal plants ..................................................................................................... 4 II.1.1 Historical use of medicinal plants ................................................................... 4 II.1.2 Phytochemistry of medicinal plants ................................................................ 4 II.2 Anacyclus pyrethrum .............................................................................................. 7 II.2.1 Presentation of plant ........................................................................................ 7 II.2.2 Taxonomic and chemical constituents ............................................................ 7 II.2.3 Biological activities ......................................................................................... 8 II.3 Cichorium intybus .................................................................................................. 9 II.3.1 Presentation of plant ........................................................................................ 9 II.3.2 Taxonomic and chemical constituents .......................................................... 10 II.3.3 Biological activities ....................................................................................... 13 II.4 Silybum marianum ............................................................................................... 14 II.4.1 Presentation of plant ...................................................................................... 14 II.4.2 Taxonomic and chemical constituents .......................................................... 15 II.4.3 Biological activities ....................................................................................... 17 PART III. MATERIAL AND METHODES .................................................................. 19 III.1 Objective of study ............................................................................................... 20 III.2 Sampling ............................................................................................................. 21 III.3 Extract Preparation.............................................................................................. 21 III.4 Phytochemical Screening .................................................................................... 21 III.4.1 Tannins ......................................................................................................... 22 III.4.2 Flavonoids .................................................................................................... 22 III.4.3 Anthocyanins ............................................................................................... 22Table of contents III.4.4 Coumarins .................................................................................................... 22 III.4.5 Alkaloids ...................................................................................................... 22 III.4.6 Saponins ....................................................................................................... 22 III.4.7 Reducing Compounds .................................................................................. 23 III.4.8 Starch ........................................................................................................... 23 III.5 DPPH Scavenging activity .................................................................................. 23 III.5.1 Principe ........................................................................................................ 23 III.5.2 Dosage.......................................................................................................... 24 III.6 Preparation of combination and statistical analysis ............................................ 24 PARTIV. RESULTS AND DISCUSSION..................................................................... 25 IV.1 Extraction Yield .................................................................................................. 26 IV.2 Qualitative Phytochemical Analysis ................................................................... 26 IV.3 Evaluation of anti-radical activity by DPPH ...................................................... 29 IV.3.1 Individual plant ............................................................................................ 29 IV.3.2 Binary mixtures ........................................................................................... 33 IV.3.3 Inhibitory concentration 50.......................................................................... 38 PART V. CONCLUSION AND PERSPECTIVES ........................................................ 39 PART VI. BIBLIOGRAPHY REFERENCES ............................................................... 41 |
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BUC-M 000469 Adobe Acrobat PDF |
